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As a positive control, we examined whether treatment with T cell-dependent antigen keyhole limpet hemocyanin (KLH) can induce activation in vitro using PBMCs depleted with CD8 + cells and CD25 High cells, hereafter referred to as CD8 − CD25 Low PBMCs, which have been used for in vitro immunogenicity assays

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87,467 movies were recorded on a K2 camera in counting mode with a total dose of 52.4 electrons per Å 2 fractionated over 40 frames (dose rate 10.48 e − /Å 2 /s) with a 0.846 Å pixel size and a defocus range between -1.2 to -3.5 μm